This detailed volume explores methods used in the development of transgenic plants. The book presents techniques for genetically altering plant tissues and cells as well as for obtaining entire transgenic plants from them. The creation of new vectors, the use of chloroplast engineering, and the most recent advances in this field, such as plant genome editing, are also covered. Written for the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Timely and practical, Transgenic Plants: Methods and Protocols serves as an ideal guide for the development of transgenic crop plants and their application for improving agriculture, environment, ecology, and health.