Cryopreservation and Freeze-Drying Protocols

This widely expanded second edition offers a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published. The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures.